A male gray bat (Myotis grisescens) was discovered on private property in Tishomingo County, northeastern Mississippi, on 20 September 2004. The endangered gray bat previously had been known only from a site known as Chalk Mine in the northeastern corner of the county, where it was last documented in 1967. The 2004 specimen was recorded approximately 42 km south of the Chalk Mine site. The discovery of this specimen is noteworthy because it represents the first gray bat documented for the state of Mississippi in 37 years.